Selective capture of native insulin antibody and its nonselective elution by a 25 μL pulse of 0.1 N HCl has been monitored at 251 and 286 nm. This technique provides a rapid, selective assay of antibodies, along with information on binding and elution that can be used for the optimization of separation and purification of Ab by affinity chromatography (see Affinity Chromatography section).
Capture and Elution of Antibodies
Binding and elution of Insulin monoclonal Ab on Sephadex-Protein A beads,
Sample volume 40 μL, 2 μg Ab/μL, carrier PBS, flow rate 1 μL/sec.
3.4.6.
